DOT Collector Training – Self-Paced Course

Categories: DOT
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About Course

This self-paced training program provides step-by-step instruction on how to properly perform Department of Transportation (DOT) urine specimen collections in compliance with 49 CFR Part 40. Designed for individuals seeking flexibility and convenience, this course allows you to learn at your own pace while gaining the essential knowledge and skills required to meet DOT standards for workplace drug testing.

What You’ll Learn:

  • The role and responsibilities of a DOT-certified collector
  • Federal regulations and updates under 49 CFR Part 40
  • The 10 Steps to Collection Site Security and Integrity
  • Proper use and completion of the Federal Custody and Control Form (CCF)
  • Handling shy bladder, temperature out-of-range, and refusal-to-test situations
  • Chain of custody procedures and common errors to avoid
  • Site preparation, privacy, and integrity protocols
  • Business operations, recordkeeping, and compliance essentials

Course Features:

  • Video lessons with real-world demonstrations
  • Downloadable forms and checklists for on-the-job use
  • Knowledge quizzes to reinforce learning
  • Printable Certificate of Completion (meets DOT collector training requirements)
  • Lifetime access to course materials for future reference

Ideal For:

  • New and aspiring DOT collectors
  • Healthcare professionals, lab techs, or mobile testing providers
  • Employers and collection sites seeking in-house training options

Training Format:
✔ 100% online
✔ Self-paced — start anytime
✔ Approximate completion time: 3–4 hours

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Course Content

DOT Overview & Regulations
Purpose: To introduce trainees to the Department of Transportation (DOT) drug and alcohol testing program, the laws that govern it, and the responsibilities of a specimen collector under 49 CFR Part 40.

  • Learning Objectives
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Module 1 Summary
DOT drug testing ensures safety in transportation sectors. Collectors are critical to maintaining the test’s integrity. All procedures must comply with 49 CFR Part 40. Errors or noncompliance can invalidate a test or trigger retraining.